I felt the need to provide a counterpoint to this.
Since I'm an introvert, I have a knack for ticking people off. Through much trial and error, I have found the best ways to ostracize myself, not only from Twitter, but pretty much any gathering of humans. I currently have 23 followers and falling, and will probably have even less after this post. Huzzah!
If you wish to retain your reclusive nature, here are the seven best ways to fail utterly at Twitter:
1) Don't care about other people. This seems obvious, but it can be deceivingly difficult to have complete apathy for other humans. (Notice I'm not talking about antipathy here. This is indifference, not disgust.) Don't take an interest in anyone else, and hopefully, they will take the hint and get their attention elsewhere.
2) Don't read tweets/posts/blogs by anyone else. People only care about themselves, and if you start reading about what they think, then they are more likely to think you care about them.
3) Never respond to anyone by name. People like attention, and if you give it to them, they might stick around, which is appalling in the grand scheme of things. In other words, if you do read what other people write, then for Pete's sake, don't tell them you did so. Though it is far better to only write about yourself, and preferably in abstract ways that mean little to anyone but yourself.
4) Talk about your cat/kids/poetry. Besides caring about themselves, people only really like controversy. Think train wrecks or beautiful people doing dumb things, and do something absolutely the opposite. I suggest linking pictures of waterfalls. Like so.
5) Never ever use "#". Using a hashtag is like an indoctrination tattoo that brands you as a member of a tribe. And tribes are made of people. And people are bad.
6) Play the Devil's advocate. Argue...I mean, debate people about everything they care about. Preferably if you can maintain a cool composure. Everyone hates a hothead troll, but people loathe the guy who never insults everyone, who reasonably shreds everything you care about to ribbons, and who always seems to be right, while making you look and feel like an idiot.
7) Never ask for the opinions of other people. If you ask for comments, that implies you will read what they write, which goes against 2).
Hopefully this post will be helpful to the frustrated introverts among us who have been doing this thing all wrong from the beginning, and will teach them the fundamental tools to sever interpersonal relationships, so that we can find fulfillment with our intrapersonal communication.